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Study on Hygiene and Sanitation and the Presence of Escherichia coli in the Tofu Industry

1Kesehatan Masyarakat, Universitas Dian Nuswantoro, Jl. Imam Bonjol No.207, Pendrikan Kidul, Kec. Semarang Tengah, Kota Semarang, Jawa Tengah 50131, Indonesia

2Kesehatan Lingkungan, Universitas Dian Nuswantoro, Jl. Imam Bonjol No.207, Pendrikan Kidul, Kec. Semarang Tengah, Kota Semarang, Jawa Tengah 50131, Indonesia

Received: 16 May 2025; Revised: 8 Aug 2025; Accepted: 11 Aug 2025; Available online: 11 Aug 2025; Published: 10 Dec 2025.
Editor(s): Marcelinus Christwardana
Open Access Copyright (c) 2025 The Author(s). Published by Centre of Biomass and Renewable Energy (CBIORE)
Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

The ineffectiveness of hygiene and sanitation standards in the food industry, including the tofu industry in Indonesia, remains a significant issue. This condition increases the risk of Escherichia coli (E. coli) contamination, which can negatively impact public health. This study aims to provide an in-depth understanding of hygiene and sanitation practices in tofu businesses in Jomblang, Semarang City, and serve as a basis for improving food safety and hygiene standards. A descriptive quantitative approach was used, with data collected through interviews, observations, and laboratory tests. The analysis revealed that many hygiene aspects, such as equipment and raw material handling, did not meet the required standards, posing a risk of microbial contamination. Laboratory tests confirmed the presence of Escherichia coli in tofu soaking water samples, indicating potential health hazards for consumers. The implementation of hygiene and sanitation principles in food production must be optimized to improve product quality and prevent the spread of infectious diseases.

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Keywords: Escherichia coli Bacteria; Hygiene and sanitation; Tofu Industry; Food safety; Microbial Quality

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