Phytochemical screening and in vitro antimicrobial activity of Rosmarinus officinalis leaves

Christophe Nsanzamahoro; Emmanuel Gatozi; Jean Damascene Urimubenshi; Jean Ntaganda

doi:10.61435/jbes.2024.19934

DOI: https://doi.org/10.61435/jbes.2024.19934

Phytochemical screening and in vitro antimicrobial activity of Rosmarinus officinalis leaves

Christophe Nsanzamahoro^{1, 2} , Emmanuel Gatozi¹, Jean Damascene Urimubenshi¹, Jean Ntaganda²

¹School of Science, University of Rwanda, Kigali, Rwanda

²School of Postgraduate Studies, Diponegoro University, Indonesia

Received: 12 Feb 2024; Revised: 27 Apr 2024; Accepted: 8 May 2024; Available online: 10 May 2024; Published: 1 Dec 2024.

Editor(s): H. Hadiyanto

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Abstract

Rosmarinus officinalis is a medicinal plant, which belongs to family Lamiaceae. It is an evergreen bush. It was analyzed for its phytochemical constitution and antibacterial activity. For this purpose, leaves of n-hexane, ethyl acetate and methanolic extracts were used. The tests of phytochemical screening showed that the plant leaves contain anthraquinones, terpenoids, flavonoids, reducing sugars, saponins, cardiac glycosides, coumarins, phenols, carbohydrates, proteins, steroids, alkaloids and quinones, while tannin was absent. The antibacterial activity was determined against E. coli and staphylococcus aureus; the invitro antibacterial activity was performed by disc diffusion method. The n-hexane and ethyl acetate extracts showed no antibacterial activity against E. coli while ethyl acetate extracts showed antibacterial activity against Staphylococcus sp. The results showed that the highest mean inhibition zone was observed in the methanolic extracts.

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Keywords: Rosmarinus officinalis; N-hexane; Ethyl acetate; Methanolic Extract; Phytochemical screening; Antibacterial activity

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In Vol 3, No 3 (2024): December 2024

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Phytochemical screening and in vitro antimicrobial activity of Rosmarinus officinalis leaves

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